Identification of Pathogens

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United States of America Patent

APP PUB NO 20090291854A1
SERIAL NO

12307524

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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Disclosed is a method for identification of microbial pathogens in a body fluid sample comprising the following steps: a) providing a body fluid sample; b) lysing the microbial pathogens and performing a nucleic acid amplification reaction on the microbial DNA encoding 16S or 18S rRNA wherein or whereafter the amplified nucleic acids are labelled; c) contacting the labelled amplified nucleic acids of step b) with a microarray comprising on defined areas on the microarray's surface immobilised probes for microbial DNA encoding 16S or 18S rRNA from microbial pathogens; d) detecting the binding of one or more species of the labelled amplified nucleic acids to a probe by detecting a labelled amplified nucleic acid being specifically bound to the microarray; and e) identifying a microbial pathogen in the body fluid sample by correlating the detected binding of the labelled amplified nucleic acids with the defined areas of the immobilised probes for microbial DNA encoding 16S or 18S rRNA from microbial pathogens.

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Patent Owner(s)

Patent OwnerAddress
AUSTRIAN RESEARCH CENTERS GMBH - ARC1010 VIENNA

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bodrossy, Levente Toltestava , HU 1 183
Nohammer, Christa Vienna , AT 4 218
Pichler, Rudolf Wampersdorf , AT 19 257
Wiesinger-Mayr, Herbert Vienna , AT 2 183

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