BIOLUMINESCENT CONSTRUCT
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United States of America Patent
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Issued Date -
Sep 24, 2009
app pub date -
Mar 19, 2009
filing date -
Mar 19, 2008
priority date (Note) -
Abandoned
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Abstract
The invention provides a construct comprising an amino acid sequence attached to a luciferin substrate, wherein the amino acid sequence is not a substrate for either luciferase or caspase-3/7 to render the substrate inactive in the presence of luciferase. The construct is useful, for example, as a negative control for in vitro apoptosis assays and the bioluminescent imaging of drug-induced apoptosis in vivo. The invention also provides methods for screening for novel modulators of an apoptosis-related disease.
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