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Abstract

The present invention provides methods and systems that acquire digital images of a microscope slide having a large variation in pixel value brightness, like, for instance, fluorescent microscope slides. The methods and the systems generate a well contrasted composite image with preserved areas of low and high fluorescent intensity from the input images. One method includes: acquiring an image of a fluorescent microscope slide at a first exposure level resulting in a first acquired image at a first contrast level; acquiring additional images of the fluorescent microscope slide each at an exposure level different from each other and different from the first exposure, resulting in different acquired images at different contrast levels; and forming a composite image of the fluorescent microscope slide by forming a weighted sum of the intensity values of the corresponding pixels of each of the images, whereby composite image has a composite contrast level over the entire composite image that represents a weighted average of the contrast levels.

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First Claim