METHOD OF SEPARATING PROTEIN, METHOD OF STAINING PROTEIN AND LIQUID PROTEIN-STAINING AGENT AND PROTEIN-STAINING KIT TO BE USED IN THESE METHODS
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United States of America Patent
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Issued Date -
Sep 10, 2009
app pub date -
Dec 6, 2005
filing date -
Dec 6, 2004
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Abandoned
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Abstract
A method of separating a protein by gel electrophoresis, which comprises a staining step for bringing a protein-containing sample into contact with a staining liquid containing a staining agent, a surfactant and a buffer solution, and an electrophoresis step for subjecting the protein-containing sample after the staining step to gel electrophoresis.According to the present invention, using a staining liquid containing a surfactant such as SDS and the like, a protein-containing sample can be stained in a short time, and can develop color with high sensitivity. In addition, since an excess staining agent migrates earlier than protein by electrophoresis, a washing operation is not necessary. Furthermore, by staining, after the first dimension electrophoresis, with a staining liquid containing a surfactant such as SDS and the like, the second dimension electrophoresis can be performed immediately. As a result, the number of steps can be reduced as compared to conventional protein separation methods, and the separation operation can be simplified. Hence, a method capable of staining and separating a protein conveniently and quickly by electrophoresis can be provided.
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