PRIMERS, METHODS AND KITS FOR AMPLIFYING OR DETECTING HUMAN LEUKOCYTE ANTIGEN ALLELES
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United States of America Patent
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Aug 22, 2008
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Oct 28, 2003
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Abstract
The present invention describes primers, methods and kits for amplifying and identifying HLA alleles. Using these primers, all HLA alleles at a single locus can be amplified using either a multiplex or non-multiplex PCR approach. Within sets of the primers, control primer pairs may be used to produce control amplicons of a predetermined size from an HLA allele only if a particular HLA locus is present in the sample. The present invention further describes primers for sequencing HLA alleles following amplification. Methods and kits for using the primers are also disclosed.
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