METHOD FOR PREDICTING THE VIABILITY AND VITALITY OF BACTERIA USABLE IN STRESSING ENVIRONMENT
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United States of America Patent
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Issued Date -
Aug 6, 2009
app pub date -
Dec 1, 2006
filing date -
Dec 2, 2005
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Abstract
A method for predicting viability, vitality and stability of bacteria, which are usable in stressing environments, produced in the form of bacterial preparations and packed in a frozen or lyophilised form, includes pre-suspending the bacterial preparations in non-stressing aqueous nutrient medium, carrying out a molecular measurement of at least one type of marker gene whose stress state is representative for each considered bacterium species, measuring intracellular pH and in comparing the measured values with reference and/or reference standard values. The use of the inventive method for controlling quality of stress adaptation of bacterial preparations and for selecting such preparations is also disclosed. The invention in particular relates to malolactic and probiotic ferments.
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