Cellular assay method for identifying pkc-0 inhibitors
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United States of America Patent
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Issued Date -
Jul 2, 2009
app pub date -
Apr 27, 2006
filing date -
May 2, 2005
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Abstract
The invention relates to a method for investigating the modulating effect of a test substance on a PKC.theta.-dependent signal transduction pathway or for finding a PKC.theta. modulator in a human or animal cell, including the steps (a) contacting the cell with the test substance or with the PKC.theta. modulator; (b) where appropriate inducing the kinase activity of PKC.theta.; (c) incubating the cell under conditions which bring about phosphorylation at least of a serine or threonine residue of PKC.theta.; (d) where appropriate lysing the cell; and (e) determining the phosphorylation content of the at least one serine or threonine residue of PKC.theta..
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