Cell free methods for detecting protein-ligand binding
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United States of America Patent
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Aug 14, 2012
Grant Date -
Jul 2, 2009
app pub date -
Nov 3, 2008
filing date -
Nov 1, 2007
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Abstract
Provided are rapid and sensitive cell-free assay methods for detecting and/or measuring specific bimolecular or higher order interactions via reassembly of a split monomeric reporter protein, and methods of detecting or identifying modulators of such interactions by the effect on the signal provided by the reassembled split reporter protein. This methodology is adaptable to protein-protein, protein-peptide, protein-nucleic acid, protein-methylated or nonmethylated nucleic acid and other small or large molecule ligands and binding proteins.
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