Ditag genome scanning technology
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United States of America Patent
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Issued Date -
May 28, 2009
app pub date -
Oct 11, 2007
filing date -
Oct 11, 2006
priority date (Note) -
Abandoned
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Abstract
The present invention provides for a method for analyzing large genomes using a process by where the genomic DNA is digested by a small base pair restriction enzyme. The fragments are then cloned and a unique ta-vector-tag is created. The tag-vector-tag fragments are purified and re-ligated to create a “ditag” library, which are then sequenced. In the final step, the sequenced ditags can be mapped back to the genome using software containing mapping algorithms and a unique ditag reference database to provide a method for scanning large portions of the genome in a reduced amount of time and cost.
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