Thermostable luciferases and methods of production
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United States of America Patent
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Issued Date -
May 28, 2009
app pub date -
Jun 12, 2007
filing date -
Sep 19, 1997
priority date (Note) -
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Abstract
Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
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