Methods of nucleic acid amplification and sequencing
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United States of America Patent
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Issued Date -
May 7, 2009
app pub date -
Jul 20, 2006
filing date -
Jul 20, 2005
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Abandoned
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Abstract
The invention relates to a method of amplifying one or more nucleic acid templates on a solid support in a nucleic acid amplification reaction, for example by solid-phase PCR using one or more amplification primers attached to the solid support. The method is characterised in that the amplification primers used comprise a template-specific portion which is a sequence of at least 26 consecutive nucleotides and are not capable of annealing to target regions in the template under conditions of the amplification reaction. The method is particularly useful for amplifying human genomic DNA.
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