NON-FLUORESCENT QUENCHER COMPOUNDS AND BIOMOLECULAR ASSAYS
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United States of America Patent
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app pub date -
Apr 4, 2008
filing date -
Aug 27, 2001
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Abandoned
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Abstract
Bis-diazo, triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides and polypeptides. The quencher moieties are non-fluorescent and accept energy transfer from fluorescent reporter labels by any energy-transfer mechanism, such as FRET.Fluorescence quencher compositions are useful in preparing quencher labeled biomolecules for various molecular biology assays based on fluorescence detection.
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