Two-color Real-time/End-point Quantitation of MicroRNAs (miRNAs)
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United States of America Patent
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Issued Date -
Apr 2, 2009
app pub date -
Dec 8, 2008
filing date -
Sep 21, 2004
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Abandoned
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Abstract
The present invention is directed to methods, reagents, kits, and compositions for detecting target polynucleotide sequences, especially small target polynucleotides such as miRNAs, between two samples. A pair of linker probes can be employed in two different reactions to query a particular species of target polynucleotide. A pair of detector probes, a single forward primer specific for the target polynucleotide, and a reverse primer can be employed in an amplification reaction to query the difference in expression level of the target polynucleotide between the two samples. In some embodiments a plurality of small miRNAs are queried with a plurality of linker probes. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.
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