Method, composition and kit for isoelectric focusing
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United States of America Patent
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May 17, 2011
Grant Date -
Jan 29, 2009
app pub date -
Jan 30, 2007
filing date -
Jan 31, 2006
priority date (Note) -
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Abstract
The present invention relates to a method, composition and a kit for isoelectric focusing. More closely, the present invention involves the use of to modified carrier ampholytes which are easy to separate from the proteins/peptides following finished focusing. The modification is that the carrier ampholytes are derivatised with handles interacting with a solid phase/matrix which makes them easy to remove and separate from the peptides after finished focusing. In this way, there is very little or no background from carrier ampholytes in the following MS-spectra.
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