METHOD FOR ISOLATING PLASMIDS FROM SUSPENDED BACTERIAL OR YEAST CELLS
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United States of America Patent
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app pub date -
Jun 4, 2007
filing date -
Mar 21, 2001
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Abandoned
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Abstract
A method for isolating plasmids from suspended bacterial or yeast cells using a filter matrix, at least one lysing substance being added to the suspension and predamaging or completely lysing the predominant portion of the suspended cells, at least one conformation-altering substance being added to the suspension to alter the conformation of the plasmids to be isolated so as to promote retention of the cell components in or at the filter matrix. The suspension is thereupon moved through the filter matrix and the cells if not yet lysed then being lysed to completion upon contact with the filter matrix and the released plasmids being retained in or at the filter matrix.
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