Method for the Production of Suitable Dna Constructs for Specific Inhibition of Gene Expression by Rna Interference
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United States of America Patent
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Issued Date -
Jan 1, 2009
app pub date -
Dec 28, 2004
filing date -
May 28, 2004
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Abstract
The invention relates to a method for the production of vectors which, following transfection thereof in eukaryotic cells, are suitable for targeted inhibition of the formation of defined proteins therein by RNA interference. The method for the production of such vectors does not include any PCR steps. It is a three-step procedure in a single reaction vessel and can be carried out within a few hours. Thus, a method is provided which allows very easy testing of a wide variety of siRNA sequences for their functionality within a very short time. Screening processes utilizing the rapid and uncomplicated production of vectors with the aid of said kit can be performed in a cost- and time-saving manner. Another advantage of vectors thus produced is their small size which, among other things, facilitates transfection.
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