Fermentation Process for Continuous Plasmid Dna Production
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United States of America Patent
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Issued Date -
Dec 25, 2008
app pub date -
Jan 31, 2007
filing date -
Feb 1, 2006
priority date (Note) -
Abandoned
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Abstract
A continuous process is described for the production of microbial plasmid DNA for use in biopharmaceutical and biotechnological applications. The process consists of: first growing microbial cells containing a plasmid at a reduced temperature in a continuous stage; followed by a second plasmid induction continuous culture stage with an increased temperature, with a residence time that allows accumulation of the plasmid product. A hold step at a reduced temperature after fermentation further increases the yield of plasmid product. The method enables production of a large quantity of highly purified plasmid DNA from a small bioreactor over time.
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