Portable Materials and Methods for Ultrasensitive Detection of Pathogen and Bioparticles
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United States of America Patent
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Issued Date -
Dec 18, 2008
app pub date -
Oct 10, 2006
filing date -
Oct 7, 2005
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Abstract
The present invention provides systems for ultrasensitive detection of pathogens and bioparticles. One embodiment of the system comprises an optical detection scheme that allows for the detection of the fluorescence signal of bacteria or other bioparticles in less than about 20 minutes. A microflow channel allows for an assay probing volume of as little as a few picoliters. In one embodiment, the system uses RuBpy dye-doped silica nanoparticles bioconjugated with specific monoclonal antibodies of the target bioparticles. The system allows for the rapid and highly sensitive and specific detection of bacteria or other bioparticles without the need for amplification or enrichment of the sample.
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