ISOLATION AND CHARACTERIZATION OF A SINGLE MITOCHONDRION
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United States of America Patent
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Issued Date -
Oct 16, 2008
app pub date -
Feb 27, 2008
filing date -
Feb 27, 2007
priority date (Note) -
Abandoned
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Abstract
A system for identifying mitochondrial heteroplasmy within eukaryotic cells is provided. This system includes means for isolating and capturing a single mitochondrion from at least one eukaryotic cell, wherein the means for isolating and capturing a single mitochondrion further includes optical tweezers or a similar optical technology; means for analyzing the isolated and captured mitochondrion, wherein the means for analyzing the isolated and captured mitochondrion further includes a DNA amplification system and a sequencing system for amplifying and sequencing DNA extracted from the mitochondrion; means for identifying at least one mitochondrial heteroplasmy of interest; and means for using the DNA amplification and DNA sequencing systems to determine the presence or absence of the mitochondrial heteroplasmy within the eukaryotic cell from which the mitochondrion was obtained.
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