PNA Probes, Kits, and Methods for Detecting Lamivudine-Resistant Hepatitis B Viruses
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United States of America Patent
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Issued Date -
Sep 25, 2008
app pub date -
Sep 25, 2007
filing date -
Mar 23, 2007
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Abstract
Disclosed are peptide nucleic acid (PNA) probes to detect lamivudine resistant mutants of hepatitis B virus (HBV), which causes acute and chronic hepatitis, kits for detecting lamivudine resistant HBV comprising the PNA probes, and methods for detecting lamivudine resistant HBV by using the kits. They can accurately detect mutations of rtL180M, rtM204V, rtM204I and rtV2071 within B and C domains of HBV DNA polymerase gene, the main cause of lamivudine resistance, as well as mixed mutants of more than one mutant. They can detect lamivudine resistant HBV with high specificity and sensitivity.
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