Method to Measure Dynamic Internal Calibration True Dose Response Curves

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United States of America Patent

APP PUB NO 20080220980A1
SERIAL NO

11632746

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Abstract

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A method of determining an amount of analyte in a sample solution is provided. The method involves the use of an assay device that has a substantially planar assay surface. The surface has a plurality of calibration dots a test dot printed thereon. The calibration dots contain pre-determined quantities of the analyte while the test dot includes a capture antibody for binding to the analyte. The analyte is mixed into a solution having a sample antibody for the analyte, where the antibody is labeled with a detectable marker. The sample solution is introduced into the loading portion of the assay device for delivery to said reading portion. The next step is to measure the intensity of detectable marker in the calibration dots. With the data obtained, one then prepares a calibration curve correlating the amount of analyte in said calibration dots to said intensity of detectable marker. The intensity of detectable marker in the test dot can be measures and the amount of analyte present in said test dot calculated by comparing the intensity of detectable marker to the amount of analyte corresponding to the intensity in said calibration curve.

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Patent Owner(s)

Patent OwnerAddress
SQI DIAGNOSTICS SYSTEMS INC F K A UMEDIK INC36 METEOR DRIVE TORONTO ONTARIO M9W 1A4

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
de, Leo Domenica Toronto, CA 1 5
Lea, Peter Toronto, CA 29 494
Liu, Jun Oakville, CA 1497 18125

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