Methods for obtaining modified DNA from a biological specimen
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United States of America Patent
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Issued Date -
Sep 4, 2008
app pub date -
Mar 3, 2008
filing date -
Mar 1, 2007
priority date (Note) -
Abandoned
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Abstract
The present invention provides a method for obtaining modified DNA from a biological specimen by obtaining a cell suspension from the specimen, if necessary; passing the cell suspension through a first filter under conditions sufficient to obtain filter-bound cells and suspended DNA; lysing the filter-bound cells under conditions sufficient to release cellular DNA; modifying the DNA bound to the filter under conditions sufficient to release the modified DNA from the filter into a flow-through volume; passing the flow-through volume through a second filter under conditions sufficient to capture the modified DNA to the second filter; and eluting the modified DNA from the second filter.
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