Phage display affinity and specificity filters and forward screen
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United States of America Patent
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app pub date -
Oct 31, 2007
filing date -
Apr 2, 2002
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Abandoned
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Abstract
The invention provides an affinity filter for the binding of phage-displayed proteins to dissolved target molecules. The phage-displayed proteins are contacted with immobilized target in the presence and absence of dissolved target; the behavior of the phage-displayed proteins as a function of concentration of dissolved target permits approximation of the affinity of the phage-displayed protein for target. The invention also provides a method to screen large numbers of compounds for their ability to compete with a compound known to bind a phage-displayed protein.
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