Process of Producing a Plastid-Targeted Protein in Plant Cells
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United States of America Patent
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Issued Date -
Jun 5, 2008
app pub date -
May 13, 2004
filing date -
May 15, 2003
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Abandoned
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Abstract
A process of producing a protein of interest in cells of a predetermined plant, comprising: introducing into said cells a vector encoding a fusion protein comprising from the N-terminus to the C-terminus (i) a transit peptide for targeting said fusion protein into plastids and, contiguous thereto, (ii) said protein of interest, wherein the C-terminal three amino acids X.sup.-3X.sup.-2X.sup.-1 of said transit peptide and the N-terminal amino acid Z of said protein of interest form a cleavage site X.sup.-3X.sup.-2X.sup.-1-Z for cleaving said fusion protein between X.sup.-1 and Z for releasing said protein of interest in plastids, whereby for a predetermined amino acid Z of said protein of interest an amino acid sequence X.sup.-3X.sup.-2X.sup.-1 is selected from the set of amino acid sequences X.sup.-3X.sup.-2X.sup.-1 naturally occurring contiguous to Z in plastid-targeted fusion proteins in plants, thereby forming said cleavage site.
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