Method for determining the amount of template nucleic acid present in a sample
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United States of America Patent
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Nov 13, 2012
Grant Date -
May 22, 2008
app pub date -
Jul 29, 2005
filing date -
Jul 29, 2004
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Abstract
A method for determining the amount of template nucleic acid present in a sample comprising the steps of: i) bringing into association with the sample all the components necessary for nucleic acid amplification, and all the components necessary for a bioluminescence assay for nucleic acid amplification and subsequently: ii) performing the nucleic acid amplification reaction; iii) monitoring the intensity of light output from the bioluminescence assay; and iv) determining the amount of template nucleic acid present in the sample.
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