Method for Detecting Conformational Change of Calmodulin, a Method for Screening a Substance Having an Activity that Affects to Conformational Change of Calmodulin
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United States of America Patent
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Issued Date -
May 22, 2008
app pub date -
Nov 7, 2005
filing date -
Nov 9, 2004
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Abandoned
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Abstract
The object of this invention is to provide a method for detecting conformational change of calmodulin easily and simply in a short period, quantitatively and in real-time manner, and to provide a method for screening a substance having an activity that affects to conformational change of calmodulin efficiently among numerous substances. To solve the object, a method is provided and the method comprising; subjecting a test sample to be tested for its activity to a sample film comprising calmodulin, detecting change(s) in tension and/or elasticity of the sample film in comparison with prior to subjecting said test sample using a mechanochemical sensor, thereby it enables real-time and short time measurement of the activity of said test sample to induce or to inhibit conformational change of calmodulin.
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