ENZYMATIC HYDROLYSIS OF STARCH
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United States of America Patent
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Issued Date -
May 1, 2008
app pub date -
Jan 3, 2007
filing date -
Oct 31, 2006
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Abstract
Provided herein are methods of increasing the enzymatic rate of hydrolysis of starch substrates. In certain embodiments the method comprises contacting a starch substrate with one or more amylase family enzymes in the presence of greater than 0.001 mM manganese ions. In certain embodiments, the method comprises contacting a starch substrate with one or more glucoamylase and/or .beta.-amylase enzymes in the presence of greater than 0.001 mM calcium ions (Ca.sup.++). In certain embodiments, the method comprises contacting the starch substrate with a .beta.-amylase in the presence of greater than 0.001 mM manganese ion, calcium ion, magnesium ion (Mg.sup.++), strontium ion (Sr.sup.++), barium ion (Ba.sup.++) or any combination of said metal ions. In certain embodiments the method comprises contacting the starch substrate with a glucoamylase in the presence of greater than 0.001 mM manganese ion, calcium ion, lithium ion (Li.sup.+), potassium ion (K.sup.+), or any combination of said metal ions. Also provided herein are compositions and kits for hydrolyzing starch.
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