METHOD FOR DETECTION OF YEAST CELL WALL
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United States of America Patent
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Issued Date -
May 1, 2008
app pub date -
Oct 27, 2006
filing date -
Oct 27, 2006
priority date (Note) -
Abandoned
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Abstract
Methods are provided for detecting the presence of a yeast cell wall in a food or animal feed suspected of containing the yeast cell wall. The method includes the steps of solubilizing the yeast cell wall, adding a lectin which preferentially binds to a mannose component of the solubilized yeast cell wall, detecting the presence of the lectin. In one embodiment, the lectin preferentially binds to an .alpha.-linked mannose component of the yeast cell wall. The .alpha.-linked mannose component may be an .alpha.-(1,3)-linked mannose or an .alpha.-(1,6)-linked mannose, and the yeast cell wall may be derived from Saccharomyces cerevisiae. Kits for accomplishing the method are described, which include a labeled lectin which preferentially binds to a mannose component of the solubilized yeast cell wall and reagents for detecting the labeled lectin.
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