Method For In Vitro Amplification Of Adult Stem Cells
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United States of America Patent
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Issued Date -
Apr 10, 2008
app pub date -
Feb 22, 2006
filing date -
Feb 28, 2005
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Abandoned
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Abstract
The present invention provides a method of efficiently expanding non-adhesive adult stem cells in vitro. More specifically, the present invention provides a method of expanding non-adhesive adult stem cells, comprising culturing non-adhesive adult stem cells in the co-presence of adhesive feeder cells and non-adhesive non-stem cells in serum-free medium, wherein the ratio of non-adhesive adult stem cell count to total cell count in the medium is kept low; non-adhesive adult stem cells obtained by the expansion method and differentiated cells thereof, and a composition comprising them; and a serum-free medium comprising a specified factor, and a kit for its preparation and the like.
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