MOLECULAR HAPLOTYPING OF GENOMIC DNA
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United States of America Patent
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Issued Date -
Mar 27, 2008
app pub date -
Jul 19, 2007
filing date -
Mar 12, 2003
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Abstract
A method of determining the haplotype structures of a nucleic acid comprising two or more single nucleotide polymorphisms (SNPs) of interest is provided. The method comprises obtaining an enriched nucleic acid fraction which comprises from 2 to 30 times more of one allelic variant of the nucleic acid than the other allelic variant of the nucleic acid, and genotyping the enriched nucleic acid fraction to identify the alleles in the two or more SNPs of interest that are present at a higher level or lower level than the other alleles in said two or more SNPs of interest, wherein the alleles that are present at a higher level are on the enriched allelic variant and form one haplotype and the alleles that are present at a lower level are on the non-enriched allelic variant and form the other haplotype. Kits for conducting the present methods are also provided.
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