Isolation of Inner Cell Mass for the Establishment of Human Embryonic Stem Cell (hESC) Lines

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United States of America Patent

APP PUB NO 20080064099A1
SERIAL NO

11939397

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Abstract

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A method of establishing a cell line from the inner cell mass of a blastocyst, comprising: isolating a blastocyst having a zona pellucida, a trophectoderm, and an inner cell mass; creating an aperture in the blastocyst by laser ablation; isolating cells from the inner cell mass from the blastocyst through the aperture; and culturing the cells to establish a cell line, wherein the cells are cultured under feeder free conditions.

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RELIANCE LIFE SCIENCES PVT LTDINDIA MAHARASHTRA

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
PARIKH, FIRUZA RAJESH Mumbai, IN 2 43
Saxena, Shailaja Anupam Mumbai, IN 2 43
Totey, Satish Mahadoerao Mumbai, IN 2 43

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