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Abstract

The invention refers to compounds according to formula (I), wherein R.sup.1 is independently in position 2', 3', or in both positions of the A ring and is hydroxyl, linear or branched -0-alkyl, wherein the alkyl group has 1-12 carbon atoms, preferably 3-8, more preferably 4 carbon --O-(alkyl)COOH wherein the alkyl group has 1-12 carbon atoms, preferably 3-8, more preferably 4 carbon atoms, --O-(aryl)COOH, -0-aryl, linear or branched --O--(CH.sub.2).sub.m--CH(NH.sub.2)--COOH with m=1-6, preferably m=2-4, or any combination thereof; R.sup.4 is in position 8', 10', or 11' of the D ring of the compound and is H or (CH.sub.2).sub.n--CH.sub.3 with n=0-6, COOH, CN, or halogen, R.sup.5 and R.sup.6 independently are ethyl-, methyl-, H, or --(CH.sub.2).sub.3 connected to position 8 or 10 of the D ring, and wherein provided that X is O, R.sup.2is F, Cl, Br, I, H, CN, --CH.dbd.CH-alkyl, --C.dbd.--C-alkyl, aryl and R.sup.3 is F, Cl, Br, I, CN, --CH.dbd.CH-alkyl, --C.dbd.C-alkyl, aryl an provided that X is S, R.sup.2 and R.sup.3 independently are F, Cl, Br, I, H, CN, --CH.dbd.CH-alkyl, --C.dbd.C-alkyl, aryl. The invention further refers to the use of the compounds according to formula (I), wherein R.sup.1, R.sup.4, R.sup.5 and R.sup.6 are defined as aforementioned and wherein R.sup.2 and R.sup.3 independently are F, Cl, Br, I, H, CN, --CH.dbd.CH-alkyl, --C.dbd.--C-alkyl, or aryl, and wherein X is O or S, as environmentally sensitive fluorescent dyes, particularly in an assay for identifying proteinlipid interactions.

First Claim