METHODS AND APPARATUS FOR GENOTYPING
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United States of America Patent
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Issued Date -
Jan 24, 2008
app pub date -
Mar 28, 2007
filing date -
Mar 29, 2006
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Abandoned
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Abstract
A method for determining the human leukocyte antigen (HLA) genotype of a nucleic acid sample, comprises: contacting a nucleic acid sample with at least one nucleic acid primer set and subjecting the mixture to a nucleic acid amplification reaction; determining the size of any amplification products produced in the amplification reaction; and correlating the presence and/or absence of specific amplification products with the presence and/or absence of specific sequence polymorphisms in the nucleic acid sample. At least one of the primer sets is a multi-specific primer set comprising at least one sequence-specific forward primer and at least one sequence-specific reverse primer and being adapted to amplify two or more specific target sequences in the nucleic acid sample. Each of the specific target sequences comprises a sequence polymorphism that is known to be associated with an HLA allele and which may be present in the nucleic acid sample to be genotyped.
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