Cell separation chip and cell culturing method using the same
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United States of America Patent
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Issued Date -
Jan 3, 2008
app pub date -
Jun 28, 2007
filing date -
Jun 28, 2006
priority date (Note) -
Abandoned
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Abstract
An inexpensive cell analysis and separation apparatus using a flow channel formed on one surface of a substrate and a chip replaceable for each sample, and a method for culturing the separated cells without contamination, are provided. A flow channel for allowing a cell-containing buffer solution to flow is provided. Cells are detected in the middle of the flow channel, and separated to a plurality of downstream flow channels based on whether each cell fulfills a predetermined condition. A culturing tank for collecting the condition-fulfilling cells is covered with a semipermeable membrane at a top surface so as to prevent contamination during cell separation. When the cell separation is finished, the flow channel communicated with the culturing tank accommodating the condition-fulfilling cells is closed, and the culturing tank is cut off from the apparatus and put into a culturing device containing a predetermined medium to culture the cells.
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