Ribosome Display or Mrna Display Method With Selection for Increased Stability of the Protein
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United States of America Patent
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Issued Date -
Dec 27, 2007
app pub date -
Jan 5, 2006
filing date -
Jan 5, 2005
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Abstract
A method of providing a subject polypeptide variant with improved stability compared with a parent subject polypeptide, employing translation and selection using an RNA expression system, wherein two or more stability selection pressures are applied simultaneously during translation, two or more stability selection pressures are applied simultaneously during selecting, or at least one stability selection pressure is applied during translation and continues to be applied during selecting, and at least one further stability selection pressure is applied during selecting.
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