Method, an Installation, and a Computer Program for Estimating the Initial Size of a Population of Nucleic Acids, in Particular by Pcr
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United States of America Patent
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app pub date -
Nov 18, 2005
filing date -
Nov 24, 2004
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Abstract
In order to estimate the size of an initial population of nucleic acids in a sample of interests, in particular by PCR, the following steps are performed: (a) providing a model of the effectiveness of the PCR, the model comprising a constant stage followed by a non-constant stage, the stages being united by a changeover region having a changeover index; (b) using the model of effectiveness to express a relationship between the changeover index and a parameter representative of the initial population size; and (c) determining the changeover index by comparison with the experimental measurements, and deducing therefrom the initial population size in the sample of interest.
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