TARGETED AND NON-TARGETED GENE INSERTIONS USING A LINEAR MINIMAL ELEMENT CONSTRUCT
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United States of America Patent
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Issued Date -
Oct 4, 2007
app pub date -
Mar 15, 2007
filing date -
Mar 15, 2006
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Abstract
The present invention provides nucleic acids and methods for disrupting genes within cells. The nucleic acids can be linear minimal elements that integrate into target cell genomes with high efficiency. The nucleic acids may be used to knock out specific genes or to increase expression of certain genes in a cell. Application in Alternaria fungi is exemplified.
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