Method of determining carbonic anhydrase i activity
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United States of America Patent
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Issued Date -
Aug 23, 2007
app pub date -
Apr 5, 2005
filing date -
Apr 5, 2004
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Abstract
To provide a method for specifically determining CA isozyme activity. The method for determining hydrolase activity of carbonic anhydrase I (CAI) in a sample, which is characterized in that the method employs, as a substrate or a combination of a substrate and an inhibitor, any of the following (A) to (E): (A) Substrate: a substrate having higher reactivity with CAI than with CAII; (B) Substrate: a substrate having higher reactivity with CAI than with CAII; Inhibitor: an inhibitor inhibiting a hydrolase other than CA, and an optional drug for enhancing inhibitory activity of the inhibitor; (C) Substrate: a substrate having higher reactivity with CAI than with CAII, and Inhibitor: a CA inhibitor inhibiting both CAI and CAII; (D) Substrate: a substrate having reactivity with both CAI and CAII, and Inhibitor: a CA inhibitor inhibiting CAI more potently than CAII; and (E) Substrate: a substrate having higher reactivity with CAI than with CAII, and Inhibitor: a CA inhibitor inhibiting CAI more potently than CAII.
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