Cell extract for high-functioned cell-free protein synthesis and method of preparing the extract
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United States of America Patent
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Issued Date -
Jun 21, 2007
app pub date -
Dec 17, 2004
filing date -
Dec 26, 2003
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Abandoned
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Abstract
The subjects of the present invention are to prepare a highly functionalized cell extract for cell-free protein synthesis and to specify and eliminate inhibitors and unstable substances present in conventional and various cell extracts for cell-free protein synthesis. Also provided is a method for preparing the cell extract for use in a cell-free protein synthesis means, wherein ATP-mediated phosphorylation pathway of sugar present in the cell extract is controlled. In particular, the control is introducing at least one selected from the following: 1) removing monosaccharides, 2) removing phosphorylated sugars, 3) controlling production of monosaccharides from polysaccharides, and 4) controlling production of phosphorylated sugars from monosaccharides.
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