Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap
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United States of America Patent
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app pub date -
Jun 22, 2006
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Oct 29, 1999
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Abstract
The invention provides compositions, kits and methods of generating a signal indicative of the presence of a target nucleic acid sequence in a sample by forming a cleavage structure. The cleavage structure is formed by incubating a sample containing a target nucleic acid with a downstream probe that forms a 3' flap when hybridized to the target. The cleavage structure is cleaved with a 3' nuclease and a detectable signal is produced.
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