Chimeric protein and its use in electron transfer methods
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United States of America Patent
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app pub date -
Jan 9, 2007
filing date -
Aug 3, 2001
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Abandoned
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Abstract
A chimeric protein comprises a redox catalytic domain from one source and an electron transfer A domain from a different source. The protein is used in a method in which a substrate for the redox catalytic domain is acted on, electrons are transferred between the redox catalytic domain and the electron transfer domain and between the electron transfer domain and an electrode. The flow of current or potential at the electrode may be monitored to determine the presence or amount of a substrate which is an analyte of interest. Alternatively current max be driven through the electrode to drive reaction of the substrate, for instance to detoxify samples. The redox catalytic domain is suitably derived from a cytochrome P450, and the electron transfer domain may be flavodoxin.
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