Enzymes and enzymic processes

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United States of America Patent

APP PUB NO 20070122865A1
SERIAL NO

10485985

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Abstract

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A chimeric protein comprises a haem domain from a mammalian or plant cytochrome P450 and a scaffold domain generally comprising an electron transfer domain, from P450 BM3 of Bacillus megaterium. The protein does not include the membrane binding portion of the wild type mammalian or plant P450 and is hence water soluble. The protein is enzymnically active on substrates for the wild-type mammalian or plant P450 and electrons are transferred to electron transfer portions for instance part of the scaffold domain, such as FAD or FMN. The protein is useful for analysing the substrate specificity of the haem domain or for detecting substrates which are analytes of interest.

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Patent Owner(s)

Patent OwnerAddress
NANOBIODESIGN LIMITED30A THAYER STREET LONDON W1U 2QP

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Gilardi, Gianfranco London, GB 7 12

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