Target nucleic acid signal detection
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United States of America Patent
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Issued Date -
May 31, 2007
app pub date -
Oct 11, 2006
filing date -
Oct 11, 2005
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Abandoned
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Abstract
Provided herein are methods, compositions and kits for detecting a target nucleic acid. A target nucleic acid can be produced, for example, by a cleavage reaction in an assay for detection of biological samples. In one aspect, a described method comprises the following steps: hybridizing the target nucleic acid having a 5' end and a 3' end to a probe to form a circular hybridization complex; forming a covalent circular target nucleic acid using the probe as a template for nucleic acid synthesis; and detecting the covalent circular target nucleic acid.
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