Compositions and methods for increasing amplification efficiency
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United States of America Patent
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Issued Date -
May 24, 2007
app pub date -
Jul 7, 2006
filing date -
Jul 7, 2005
priority date (Note) -
Abandoned
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Abstract
Compositions and methods are provided that improve the specificity and efficiency of nucleic acid amplification. A binding partner may be bound to a DNA polymerase enzyme where the binding partner substantially inhibits the activity of the polymerase. A second enzyme modifies the binding partner in a manner that relieves the inhibition of the polymerase activity. The activity of the second enzyme may be inhibited in a temperature-sensitive manner such that the second enzyme is active only at elevated temperatures. As a consequence, the polymerase enzyme also is active only when the temperature is elevated.
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