Detection of intracellular enzyme complex
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United States of America Patent
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Issued Date -
May 10, 2007
app pub date -
Oct 24, 2006
filing date -
Oct 24, 2005
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Abandoned
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Abstract
Methods and compositions are provided for determining intracellular translocation of proteins employing .beta.-galactosidase fragments that independently complex to form an active enzyme. Cells are used having two fusion constructs: one fragment bound to a protein of interest; and the other fragment bound to a compartment localizing signal. The cells are used to screen compounds for their effect on translocation, where a substrate containing high ionic strength solution is used for detection of the enzyme complex.
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