Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry
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United States of America Patent
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Aug 3, 2006
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Nov 14, 2001
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Abstract
The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken from multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.
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