METHOD AND PROCESS OF GENETIC TRANSFORMATION USING SUPERCRITICAL FLUIDS
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United States of America Patent
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Issued Date -
Feb 22, 2007
app pub date -
Jun 14, 2006
filing date -
Aug 22, 2005
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Abandoned
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Abstract
Methods are provided for improving the ability of non-naturally transformable cells to take up and integrate DNA. Generally, the invention provides a method for transforming cells, comprising combining deoxyribonucleic acid (DNA) and a recipient cell culture for the uptake of the DNA, placing mixture of recipient cell culture and DNA into a vessel, injecting a supercritical fluid into the vessel, removing the recipient cells from the vessel, and placing removed cells into a growth media with selective conditions to allow expression of transformed DNA.
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