Identification and characterization of analytes from whole blood
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United States of America Patent
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app pub date -
Jun 19, 2006
filing date -
Apr 15, 2002
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Abstract
The invention provides a method of identifying and detecting targets in blood samples by binding to multiple ligands. The method comprises providing ligands attached to a support, and contacting the ligands with targets in blood to allow at least one target to bind to at least one ligand. The method further comprises removal of non-bound targets and cellular components such as blood cells, platelets, abundant plasma proteins followed by dissociation and elution of the target(s). The eluted targets are detected by a variety of means in concentrations which are a function of their presence in one sample as compared with their concentration in a second sample and are simultaneously enriched for trace components.
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