Nucleic acid extraction method
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United States of America Patent
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app pub date -
Apr 7, 2006
filing date -
Oct 15, 2003
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Abandoned
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Abstract
The invention relates to a method for the extraction of nucleic acids from microorganisms and to the use thereof in a method for the analysis of a microbial population in a given environment, e.g. a population of microorganisms taken from the air. In particular, the invention relates to an extraction method in which the following three lysis steps are performed successively in any order, namely: (i) chemical lysis of the microorganisms, consisting in bringing the microorganisms into contact with a lysis solution containing a detergent; (ii) thermal shock lysis comprising the incubation of the extract at a temperature lower than 0.degree. C., immediately followed by the incubation of the extract at a temperature of at least 95.degree. C. and preferably 100.degree. C.; and mechanical lysis in which the extract is stirred in the presence of balls.
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