Ex vivo expansion of primary animal cells
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United States of America Patent
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Issued Date -
Jan 11, 2007
app pub date -
Jun 28, 2006
filing date -
Jun 29, 2005
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Abandoned
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Abstract
An ex vivo method of expanding animal cells whose differentiation state is controllable by modulating TGF-.beta. signaling includes the steps of: (a) providing an animal subject having cells with a first phenotype; (b) isolating the cells from the animal subject; (c) placing the cells in an ex vivo culture system including a culture vessel having at least one surface and a medium in contact with the at least one surface, the medium being essentially free of intact amniotic membrane and feeder cells; and (d) culturing the cells in the medium under conditions which downregulate TGF-.beta. signaling in the cells and allow the cells to proliferate while maintaining the first phenotype.
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